Sergei Zolotukhin

Sergei Zolotukhin, Ph.D.

Professor

Department: MD-PEDS-CELL / MOLEC THERP DIV
Business Phone: (352) 273-8150
Business Email: szlt@ufl.edu

About Sergei Zolotukhin

Dr. Zolotukhin, PhD, is Professor of Pediatrics at University of Florida, a graduate of Kyiv State University (Ukraine). He is leading a research in Directed Evolution of combinatorial AAV capsid libraries, tissue-specific targeting of rAAV, and development of novel platforms for a large-scale rAAV manufacturing.

Publications

2021
A comprehensive study of a 29-capsid AAV library in a non-human primate central nervous system.
Molecular therapy : the journal of the American Society of Gene Therapy. 29(9):2806-2820 [DOI] 10.1016/j.ymthe.2021.07.010. [PMID] 34298128.
2021
Applying machine learning to predict viral assembly for adeno-associated virus capsid libraries.
Molecular therapy. Methods & clinical development. 20:276-286 [DOI] 10.1016/j.omtm.2020.11.017. [PMID] 33511242.
2021
Mixture interactions at mammalian olfactory receptors are dependent on the cellular environment.
Scientific reports. 11(1) [DOI] 10.1038/s41598-021-88601-0. [PMID] 33927269.
2021
Scratching the surface of RGD-directed AAV capsid engineering.
Molecular therapy : the journal of the American Society of Gene Therapy. 29(11):3099-3100 [DOI] 10.1016/j.ymthe.2021.10.020. [PMID] 34699781.
2021
The CRTC1-MAML2 fusion is the major oncogenic driver in mucoepidermoid carcinoma.
JCI insight. 6(7) [DOI] 10.1172/jci.insight.139497. [PMID] 33830080.
2021
Y1 receptors modulate taste-related behavioral responsiveness in male mice to prototypical gustatory stimuli.
Hormones and behavior. 136 [DOI] 10.1016/j.yhbeh.2021.105056. [PMID] 34509673.
2020
Engineering and In Vitro Selection of a Novel AAV3B Variant with High Hepatocyte Tropism and Reduced Seroreactivity.
Molecular therapy. Methods & clinical development. 19:347-361 [DOI] 10.1016/j.omtm.2020.09.019. [PMID] 33145371.
2019
Achieving High-Yield Production of Functional AAV5 Gene Delivery Vectors via Fedbatch in an Insect Cell-One Baculovirus System.
Molecular therapy. Methods & clinical development. 13:279-289 [DOI] 10.1016/j.omtm.2019.02.003. [PMID] 30886878.
2019
Removal of Endotoxin from rAAV Samples Using a Simple Detergent-Based Protocol.
Molecular therapy. Methods & clinical development. 15:112-119 [DOI] 10.1016/j.omtm.2019.08.013. [PMID] 31649960.
2019
Taste Receptor Cells in Mice Express Receptors for the Hormone Adiponectin.
Chemical senses. 44(6):409-422 [DOI] 10.1093/chemse/bjz030. [PMID] 31125082.
2018
Helper-free Production of Laboratory Grade AAV and Purification by Iodixanol Density Gradient Centrifugation.
Molecular therapy. Methods & clinical development. 10:1-7 [DOI] 10.1016/j.omtm.2018.05.001. [PMID] 30073177.
2017
An Immune-Competent Murine Model to Study Elimination of AAV-Transduced Hepatocytes by Capsid-Specific CD8+ T Cells.
Molecular therapy. Methods & clinical development. 5:142-152 [DOI] 10.1016/j.omtm.2017.04.004. [PMID] 28480313.
2017
Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human versus Insect Cells.
Molecular therapy : the journal of the American Society of Gene Therapy. 25(12):2661-2675 [DOI] 10.1016/j.ymthe.2017.08.003. [PMID] 28890324.
2017
Evaluation of engineered AAV capsids for hepatic factor IX gene transfer in murine and canine models.
Journal of translational medicine. 15(1) [DOI] 10.1186/s12967-017-1200-1. [PMID] 28460646.
2017
OneBac 2.0: Sf9 Cell Lines for Production of AAV1, AAV2, and AAV8 Vectors with Minimal Encapsidation of Foreign DNA.
Human gene therapy methods. 28(1):15-22 [DOI] 10.1089/hgtb.2016.164. [PMID] 28125901.
2016
Altering Tropism of rAAV by Directed Evolution.
Methods in molecular biology (Clifton, N.J.). 1382:151-73 [DOI] 10.1007/978-1-4939-3271-9_11. [PMID] 26611585.
2016
Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa.
PloS one. 11(5) [DOI] 10.1371/journal.pone.0154779. [PMID] 27144303.
2016
Prader-Willi Critical Region, a Non-Translated, Imprinted Central Regulator of Bone Mass: Possible Role in Skeletal Abnormalities in Prader-Willi Syndrome.
PloS one. 11(1) [DOI] 10.1371/journal.pone.0148155. [PMID] 26824232.
2016
Snord116 is critical in the regulation of food intake and body weight.
Scientific reports. 6 [DOI] 10.1038/srep18614. [PMID] 26726071.
2015
Adeno-Associated Virus Type 2 and Hepatocellular Carcinoma?
Human gene therapy. 26(12):779-81 [DOI] 10.1089/hum.2015.29014.kib. [PMID] 26690810.
2015
E Pluribus Unum: 50 Years of Research, Millions of Viruses, and One Goal–Tailored Acceleration of AAV Evolution.
Molecular therapy : the journal of the American Society of Gene Therapy. 23(12):1819-31 [DOI] 10.1038/mt.2015.173. [PMID] 26388463.
2015
High-accuracy biodistribution analysis of adeno-associated virus variants by double barcode sequencing
. 2
2015
High-accuracy biodistribution analysis of adeno-associated virus variants by double barcode sequencing.
Molecular therapy. Methods & clinical development. 2 [DOI] 10.1038/mtm.2015.41. [PMID] 26793739.
2015
OneBac 2.0: Sf9 Cell Lines for Production of AAV5 Vectors with Enhanced Infectivity and Minimal Encapsidation of Foreign DNA.
Human gene therapy. 26(10):688-97 [DOI] 10.1089/hum.2015.050. [PMID] 26134901.
2014
A simplified purification protocol for recombinant adeno-associated virus vectors.
Molecular therapy. Methods & clinical development. 1 [DOI] 10.1038/mtm.2014.34. [PMID] 26015974.
2014
Identification of low-expressing transcripts of the NPY receptors’ family in the murine lingual epithelia.
Methods in molecular biology (Clifton, N.J.). 1211:213-28 [DOI] 10.1007/978-1-4939-1459-3_17. [PMID] 25218388.
2014
Neuropeptide Y Attenuates Stress-Induced Bone Loss Through Suppression of Noradrenaline Circuits
Journal of Bone and Mineral Research. 29(10):2238-2249 [DOI] 10.1002/jbmr.2205. [PMID] 24535841.
2014
OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy.
Human gene therapy. 25(3):212-22 [DOI] 10.1089/hum.2013.184. [PMID] 24299301.
2014
Vector design Tour de Force: integrating combinatorial and rational approaches to derive novel adeno-associated virus variants
. 22(11):1900-1909
2014
Vector design Tour de Force: integrating combinatorial and rational approaches to derive novel adeno-associated virus variants.
Molecular therapy : the journal of the American Society of Gene Therapy. 22(11):1900-9 [DOI] 10.1038/mt.2014.139. [PMID] 25048217.
2013
Directed Evolution of Aav Capsid for Targeted Cancer Therapy
Molecular Therapy. 21
2013
Metabolic hormones in saliva: origins and functions.
Oral diseases. 19(3):219-29 [DOI] 10.1111/odi.12015. [PMID] 22994880.
2013
Modulation of taste responsiveness by the satiation hormone peptide YY.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 27(12):5022-33 [DOI] 10.1096/fj.13-228064. [PMID] 24043261.
2013
Salivary peptide tyrosine-tyrosine 3-36 modulates ingestive behavior without inducing taste aversion.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(47):18368-80 [DOI] 10.1523/JNEUROSCI.1064-13.2013. [PMID] 24259562.
2013
Structural insights into adeno-associated virus serotype 5.
Journal of virology. 87(20):11187-99 [DOI] 10.1128/JVI.00867-13. [PMID] 23926356.
2012
Design and Characterization of New Generation Synthetic Aav Capsid Libraries
Molecular Therapy. 20
2012
Development of Novel Recombinant AAV Vectors and Strategies for the Potential Gene Therapy of Hemophilia.
Journal of genetic syndromes & gene therapy. S1 [PMID] 23264889.
View on: PubMed
2012
Distribution of Y-receptors in murine lingual epithelia.
PloS one. 7(9) [DOI] 10.1371/journal.pone.0046358. [PMID] 23050020.
2012
Enhanced Fracture Healing Utilizing in Vivo Biopanning of Adeno-Associated Virus From a Directed Evolution Library
Molecular Therapy. 20
2012
High-efficiency transduction of human monocyte-derived dendritic cells by capsid-modified recombinant AAV2 vectors.
Vaccine. 30(26):3908-17 [DOI] 10.1016/j.vaccine.2012.03.079. [PMID] 22497875.
2012
Neutralizing antibodies against AAV serotypes 1, 2, 6, and 9 in sera of commonly used animal models.
Molecular therapy : the journal of the American Society of Gene Therapy. 20(1):73-83 [DOI] 10.1038/mt.2011.177. [PMID] 21915102.
2012
Structural insight into the unique properties of adeno-associated virus serotype 9.
Journal of virology. 86(12):6947-58 [DOI] 10.1128/JVI.07232-11. [PMID] 22496238.
2012
The 3D Structure of Adeno-Associated Virus Serotype 9 and Its Unique Properties
Molecular Therapy. 20
2012
Y1 and Y5 Receptors Are Both Required for the Regulation of Food Intake and Energy Homeostasis in Mice
PLoS One. 7(6) [DOI] 10.1371/journal.pone.0040191. [PMID] 22768253.
2011
Salivary PYY: a putative bypass to satiety.
PloS one. 6(10) [DOI] 10.1371/journal.pone.0026137. [PMID] 22028819.
2010
An animal model of PDH deficiency using AAV8-siRNA vector-mediated knockdown of pyruvate dehydrogenase E1╬▒.
Molecular genetics and metabolism. 101(2-3):183-91 [DOI] 10.1016/j.ymgme.2010.07.008. [PMID] 20685142.
2010
High-efficiency transduction and correction of murine hemophilia B using AAV2 vectors devoid of multiple surface-exposed tyrosines.
Molecular therapy : the journal of the American Society of Gene Therapy. 18(12):2048-56 [DOI] 10.1038/mt.2010.172. [PMID] 20736929.
2010
The Role of Salivary Pyy3-36 in Food Intake and Behavior – An Alternative Pathway for Satiety
American Journal of Gastroenterology. 105
2009
A Novel Inducible System for Highly Efficient Production of Recombinant Adeno-Associated Virus (Raav) Vectors in Insect Sf9 Cells
Molecular Therapy. 17
2009
An inducible system for highly efficient production of recombinant adeno-associated virus (rAAV) vectors in insect Sf9 cells.
Proceedings of the National Academy of Sciences of the United States of America. 106(13):5059-64 [DOI] 10.1073/pnas.0810614106. [PMID] 19279219.
2009
High-Efficiency Transduction of Human Cells in Vitro and Murine Hepatocytes in Vivo At Further Reduced Doses of Aav2 Vectors Containing Multiple-Mutations in Surface-Exposed Tyrosine Residues in the Viral Capsid
Molecular Therapy. 17
2009
High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors.
Molecular therapy : the journal of the American Society of Gene Therapy. 17(3):463-71 [DOI] 10.1038/mt.2008.269. [PMID] 19066593.
2009
Modulation of Long-Term Feeding Behavior and Social Interactions Using Non-Invasive Gene Therapy With Recombinant Aav Vectors
Molecular Therapy. 17
2009
Novel Tyrosine Mutant Vectors Expand the Utilities of Aav-Mediated Muscle Gene Therapy
Molecular Therapy. 17
2009
Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9.
Acta crystallographica. Section F, Structural biology and crystallization communications. 65(Pt 7):715-8 [DOI] 10.1107/S1744309109021460. [PMID] 19574648.
2009
Structural Studies of Adeno-Associated Virus Serotypes Towards Improved Gene Therapy Applications
Molecular Therapy. 17
2009
Towards Characterizing Vector-Host Interactions: Adeno-Associated Virus Capsids in Complex With Antibody Fragments
Molecular Therapy. 17
2008
Conditional, genetic disruption of ciliary neurotrophic factor receptors reveals a role in adult motor neuron survival.
The European journal of neuroscience. 27(11):2830-7 [DOI] 10.1111/j.1460-9568.2008.06298.x. [PMID] 18588528.
2008
Next generation of adeno-associated virus 2 vectors: point mutations in tyrosines lead to high-efficiency transduction at lower doses.
Proceedings of the National Academy of Sciences of the United States of America. 105(22):7827-32 [DOI] 10.1073/pnas.0802866105. [PMID] 18511559.
2008
Optimization of recombinant adeno-associated viral vectors for human beta-globin gene transfer and transgene expression.
Human gene therapy. 19(4):365-75 [DOI] 10.1089/hum.2007.173. [PMID] 18399730.
2008
Pres 30-Locating the Vp1 Structural Protein in Adeno-Associated Viruses
. 235
2008
Pres 33-Ph Induced Conformational Changes On the Surface of the Aav1 Capsid: Implications for Capsid Trafficking and Uncoating
. 235
2008
Synergy between leptin therapy and a seemingly negligible amount of voluntary wheel running prevents progression of dietary obesity in leptin-resistant rats.
Diabetes. 57(3):614-22 [PMID] 18086903.
View on: PubMed
2008
Tyrosine-phosphorylation of AAV2 vectors and its consequences on viral intracellular trafficking and transgene expression.
Virology. 381(2):194-202 [DOI] 10.1016/j.virol.2008.08.027. [PMID] 18834608.
2007
A dual role of EGFR protein tyrosine kinase signaling in ubiquitination of AAV2 capsids and viral second-strand DNA synthesis.
Molecular therapy : the journal of the American Society of Gene Therapy. 15(7):1323-30 [PMID] 17440440.
View on: PubMed
2007
Ectopic expression of Wnt10b decreases adiposity and improves glucose homeostasis in obese rats.
American journal of physiology. Endocrinology and metabolism. 293(3):E726-36 [PMID] 17578883.
View on: PubMed
2007
Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7.
Acta crystallographica. Section F, Structural biology and crystallization communications. 63(Pt 12):1073-6 [PMID] 18084098.
View on: PubMed
2007
Structure of adeno-associated virus serotype 8, a gene therapy vector.
Journal of virology. 81(22):12260-71 [PMID] 17728238.
View on: PubMed
2006
Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 1.
Acta crystallographica. Section F, Structural biology and crystallization communications. 62(Pt 12):1271-4 [PMID] 17142915.
View on: PubMed
2005
Efficient transduction of vascular endothelial cells with recombinant adeno-associated virus serotype 1 and 5 vectors.
Human gene therapy. 16(2):235-47 [PMID] 15761263.
View on: PubMed
2005
Gene therapy for obesity.
Expert opinion on biological therapy. 5(3):347-57 [PMID] 15833072.
View on: PubMed
2005
Production of recombinant adeno-associated virus vectors.
Human gene therapy. 16(5):551-7 [PMID] 15916480.
View on: PubMed
2005
Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8.
Acta crystallographica. Section F, Structural biology and crystallization communications. 61(Pt 6):558-61 [PMID] 16511095.
View on: PubMed
2005
Production, purification, crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 5.
Acta crystallographica. Section F, Structural biology and crystallization communications. 61(Pt 10):917-21 [PMID] 16511195.
View on: PubMed
2005
Successful production of pseudotyped rAAV vectors using a modified baculovirus expression system.
Molecular therapy : the journal of the American Society of Gene Therapy. 12(6):1217-25 [PMID] 16213797.
View on: PubMed
2004
Adeno-associated virus type 2 VP2 capsid protein is nonessential and can tolerate large peptide insertions at its N terminus.
Journal of virology. 78(12):6595-609 [PMID] 15163751.
View on: PubMed
2004
Differential modulation of energy balance by leptin, ciliary neurotrophic factor, and leukemia inhibitory factor gene delivery: microarray deoxyribonucleic acid-chip analysis of gene expression.
Endocrinology. 145(4):2035-45 [PMID] 14715713.
View on: PubMed
2004
Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system.
Molecular therapy : the journal of the American Society of Gene Therapy. 10(2):302-17 [PMID] 15294177.
View on: PubMed
2003
Central leptin gene therapy fails to overcome leptin resistance associated with diet-induced obesity.
American journal of physiology. Regulatory, integrative and comparative physiology. 285(5):R1011-20 [PMID] 14557234.
View on: PubMed
2003
Identification of amino acid residues in the capsid proteins of adeno-associated virus type 2 that contribute to heparan sulfate proteoglycan binding.
Journal of virology. 77(12):6995-7006 [PMID] 12768018.
View on: PubMed
2003
Leptin-induced leptin resistant rats exhibit enhanced responses to the melanocortin agonist MT II.
Neuropharmacology. 45(2):211-9 [PMID] 12842127.
View on: PubMed
2003
Sustained peripheral expression of transgene adiponectin offsets the development of diet-induced obesity in rats.
Proceedings of the National Academy of Sciences of the United States of America. 100(24):14217-22 [PMID] 14617771.
View on: PubMed
2002
Central leptin gene delivery evokes persistent leptin signal transduction in young and aged-obese rats but physiological responses become attenuated over time in aged-obese rats.
Neuropharmacology. 42(4):548-61 [PMID] 11955525.
View on: PubMed
2002
Leptin-induced leptin resistance reveals separate roles for the anorexic and thermogenic responses in weight maintenance.
Endocrinology. 143(8):3026-35 [PMID] 12130569.
View on: PubMed
2002
Production and purification of serotype 1, 2, and 5 recombinant adeno-associated viral vectors.
Methods (San Diego, Calif.). 28(2):158-67 [PMID] 12413414.
View on: PubMed
2002
Regulation of adiponectin and leptin gene expression in white and brown adipose tissues: influence of beta3-adrenergic agonists, retinoic acid, leptin and fasting.
Biochimica et biophysica acta. 1584(2-3):115-22 [PMID] 12385894.
View on: PubMed
2002
Streamlined large-scale production of recombinant adeno-associated virus (rAAV) vectors.
Methods in enzymology. 346:413-30 [PMID] 11883083.
View on: PubMed
2002
Transduction of human and mouse pancreatic islet cells using a bicistronic recombinant adeno-associated viral vector.
Molecular therapy : the journal of the American Society of Gene Therapy. 5(2):154-60 [PMID] 11829522.
View on: PubMed
2001
Central leptin gene therapy suppresses body weight gain, adiposity and serum insulin without affecting food consumption in normal rats: a long-term study.
Regulatory peptides. 99(2-3):69-77 [PMID] 11384767.
View on: PubMed
2000
Long-term differential modulation of genes encoding orexigenic and anorexigenic peptides by leptin delivered by rAAV vector in ob/ob mice. Relationship with body weight change.
Regulatory peptides. 92(1-3):97-105 [PMID] 11024571.
View on: PubMed
2000
Production and purification of recombinant adeno-associated virus.
Methods in enzymology. 316:743-61 [PMID] 10800712.
View on: PubMed
1999
Long-term actions of vector-derived nerve growth factor or brain-derived neurotrophic factor on choline acetyltransferase and Trk receptor levels in the adult rat basal forebrain.
Neuroscience. 90(3):815-21 [PMID] 10218782.
View on: PubMed
1999
Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield.
Gene therapy. 6(6):973-85 [PMID] 10455399.
View on: PubMed
1998
A fluorescence video-endoscopy technique for detection of gene transfer and expression.
Gene therapy. 5(2):166-73 [PMID] 9578835.
View on: PubMed
1998
Neuron-specific transduction in the rat septohippocampal or nigrostriatal pathway by recombinant adeno-associated virus vectors.
Experimental neurology. 150(2):183-94 [PMID] 9527887.
View on: PubMed
1997
Efficient transduction of green fluorescent protein in spinal cord neurons using adeno-associated virus vectors containing cell type-specific promoters.
Gene therapy. 4(1):16-24 [PMID] 9068791.
View on: PubMed
1996
A “humanized” green fluorescent protein cDNA adapted for high-level expression in mammalian cells.
Journal of virology. 70(7):4646-54 [PMID] 8676491.
View on: PubMed
1996
Sequence requirements for binding of Rep68 to the adeno-associated virus terminal repeats.
Journal of virology. 70(3):1542-53 [PMID] 8627673.
View on: PubMed

Grants

Jul 2021 ACTIVE
Task Order 14 – Directed Evolution of AAV Combinatorial Capsid Libraries to Derive Human Targeted Capsids
Role: Principal Investigator
Funding: AAVANTIBIO
Jul 2020 ACTIVE
Artificial intelligence-directed approach in human tissue manufacturing for therapeutic targeting
Role: Co-Investigator
Funding: SAREPTA THERAPEUTICS
Mar 2020 – Sep 2020
Aavanti Task Order #2 – Molecular biology support for development of rAAV vectors for SARS-CoV2 Vaccine
Role: Principal Investigator
Funding: AAVANTIBIO
Sep 2019 ACTIVE
Engineering AAV for safe and efficient gene delivery to the human retina
Role: Co-Investigator
Funding: NATL INST OF HLTH NEI
Sep 2019 ACTIVE
STTR – Phase 2 – Targeting glioblastoma stem-like cells with custom-designed viral vectors
Role: Principal Investigator
Funding: LACERTA THERAPEUTICS via NATL INST OF HLTH NCI
Aug 2019 ACTIVE
Development of a Novel AAV Vector Capsid Optimized for OA Gene Therapy
Role: Co-Investigator
Funding: US ARMY MED RES ACQUISITION
Apr 2018 – Jun 2019
STTR – Targeting glioblastoma stem-like cells with custom-designed viral vectors
Role: Principal Investigator
Funding: LACERTA THERAPEUTICS via NATL INST OF HLTH NCI
Aug 2017 ACTIVE
Optimizing AAV Vectors for Central Nervous System transduction
Role: Co-Investigator
Funding: NATL INST OF HLTH NINDS
Jul 2017 – Dec 2019
Use of barcoded genomes to determine the frequency of transduction and gene expression of approximately 34 serotypes/mutants of rAAV in various regions of the primate brain
Role: Principal Investigator
Funding: LACERTA THERAPEUTICS
May 2017 ACTIVE
Therapy development for open-angle glaucoma
Role: Project Manager
Funding: MICHIGAN STATE UNIV via NATL INST OF HLTH NEI
Feb 2017 – May 2018
Characterization of salivary adiponectin and its effect on taste perception
Role: Other
Funding: NATL INST OF HLTH NIDCD
Dec 2015 – Nov 2017
AAV-mediated optogenetic gene therapy in bipolar cells
Role: Project Manager
Funding: APPLIED GENETICS TECH CORP
May 2015 – Apr 2020
NEXT GENERATION OF RECOMBINANT AAV SEROTYPE VECTORS FOR GENE THERAPY
Role: Project Manager
Funding: NATL INST OF HLTH NHLBI
Sep 2014 – Aug 2018
AAV Capsid Library Screening
Role: Principal Investigator
Funding: APPLIED GENETICS TECH CORP
Aug 2014 – May 2020
Signaling and targeting of CRTC1-MAML2 fusion oncoprotein in salivary gland tumor
Role: Project Manager
Funding: NATL INST OF HLTH
Apr 2014 – May 2019
Developing efficient AAV vectors for photoreceptor targeting via the vitreous
Role: Project Manager
Funding: NATL INST OF HLTH NEI
Jul 2013 – Jun 2020
Modulation of Taste-Related Behavior by Molecular Mediators of Appetite & Satiety
Role: Project Manager
Funding: NATL INST OF HLTH NIDCD

Patents

Issued July 2018
Capsid-Modified rAAV Vector Compositions and Methods Therefor
#10,011,640
Issued March 2018
Activation of the NF-kB Pathway by AAV Vectors and its Implications in Immune Response and Gene Therapy-CIP
#9,920,097
Published February 2018
Capsid-Modified rAAV Vector Compositions Having Improved Transduction Efficiencies, and Methods of Use (CON)
#US-2018-0244727
Issued October 2017
Capsid-Modified rAAV Vectors and Methods of Use (CON)
#9,775,918
Published August 2017
Capsid-Modified rAAV Vectors and Methods of Use (CON)
#US2018/0036428
Published April 2017
Expression of PYY and Excendin-4 in Murine Saliva Promotes Weight Loss
#US2017/0326257
Issued November 2016
Satiation Gut Peptides Gene Therapy for Obesity
#9,492,505
Published September 2016
Satiation Peptide Administration
#US2017/0080056
Issued October 2015
Improved Transduction Efficiency, Capsid-Modified RAAV Vectors and Methods of Use (CON)
#9,157,098
Published September 2014
Synthetic Combinatorial AAV Capsid Library for Targeted Gene Therapy
#2016/0369298
Issued August 2014
Therapeutic Methods Based on Tyrosine-Substituted, Capsid-Modified RAAV Vectors (DIV)
#8,802,440
Published May 2014
Capsid-Modified rAAV3 Vector Compositions and Uses in Gene Therapy of Human Liver Cancer (CIP)
#US2016/0333372
Issued March 2014
Stable Insect Cell Lines for Production of rAAV Vectors
#8,679,837
Issued May 2013
Tyrosine-Modified Recombinant AAV Vectors for Human Gene Therapy
#8,445,267
Issued May 2007
Modified AAV
#7,220,577
Issued August 2006
Production of Pseudotyped Recombinant AAV Virions
#7,094,604
Published January 2005
A Modified Baculovirus Expression System for Production of Pseudotyped rAAV Vector
#US-2006-0166363-A1
Issued August 2004
Methods for Large-Scale Production of Recombinant AAV Vectors
#6,783,972
Issued December 2003
Method of Preparing Recombinant Adeno-Associated Virus Compositions
#6,660,514
Published December 2003
Methods of Preparing Recombinant Adeno-Associated Virus Compositions
#2004/0121444-A1
Published June 2003
Production of Pseudotyped Recombinant AAV Virions
#2007-0015238-A1
Published January 2003
Adiponectin Gene Therapy
#US-2003-0147855-A1
Issued November 2000
Method of Preparing Recombinant AAV Compositions
#6,146,874
Issued February 2000
Humanized Green Fluorescent Protein Genes and Methods
#6,020,192
Issued October 1999
Humanized Green Fluorescent Protein Genes and Methods
#5,968,750
Issued February 1999
Humanized Green Fluorescent Protein Genes And Methods
#5,874,304

Teaching Profile

Courses Taught
2014,2016-2018,2018-2021,2020-2022
GMS6251 Molecular Therapy I ? Vectors and Molecular Mechanisms
2017-2021
GMS6036 Molecular Virology III: DNA Viruses
2018
GMS7980 Research for Doctoral Dissertation
2018
BSC4913 Independent Research in Bioinformatics
2012
GMS5905 Special Topics in Biomedical Sciences
2009
ALS4905 Problems in Agricul

Contact Details

Phones:
Business:
(352) 273-8150
Emails:
Business:
szlt@ufl.edu