Selected Publications

  1. Kondratova L, Kondratov O, Ragheb R, Zolotukhin S. Removal of Endotoxin From rAAV Samples Using a Simple Detergent-Based Protocol. Mol Ther Methods Clin Dev. 2019;15:112‐119. Published 2019 Sep 6. doi:10.1016/j.omtm.2019.08.013
  2. Joshi PRH, Cervera L, Ahmed I, Kondratov O, Zolotukhin S, Schrag J, Chahal PS, Kamen A. Achieving high yield production of functional AAV5 gene delivery vectors via fedbatch in insect cell/one baculovirus system. Mol Ther Methods Clin Dev. 2019;13:279‐289. Published 2019 Feb 16. doi:10.1016/j.omtm.2019.02.003.
  3. Crosson SM, Dib P, Smith JK, Zolotukhin S. Helper-free Production of Laboratory Grade AAV and Purification by Iodixanol Density Gradient Centrifugation. Mol Ther Methods Clin Dev. 2018;10:1‐7. Published 2018 May 8. doi:10.1016/j.omtm.2018.05.001
  4. Kondratov O, Marsic D, Crosson SM, Mendez-Gomez HR, Moskalenko O, Mietzsch M, Heilbronn R, Allison JR, Green KB, Agbandje-McKenna M, Zolotukhin S. Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human versus Insect Cells. Mol Ther. 2017;25(12):2661‐2675. doi:10.1016/j.ymthe.2017.08.003
  5. Mietzsch M, Casteleyn V, Weger S, Zolotukhin S, Heilbronn R. OneBac 2.0: Sf9 Cell Lines for Production of AAV5 Vectors with Enhanced Infectivity and Minimal Encapsidation of Foreign DNA. Hum Gene Ther. 2015 Oct;26(10):688-97.
  6. Grimm D, Zolotukhin S. E Pluribus Unum: 50 Years of Research, Millions of Viruses, and One Goal-Tailored Acceleration of AAV Evolution. Mol Ther. 2015 Dec;23(12):1819-31.
  7. Marsic D, Zolotukhin S. Altering Tropism of rAAV by Directed Evolution. Methods Mol Biol. 2016;1382:151-73.
  8. Marsic D, Méndez-Gómez HR, Zolotukhin S. High-accuracy biodistribution analysis of adeno-associated virus variants by double barcode sequencing. Mol Ther Methods Clin Dev. 2015 Oct 28;2:15041.
  9. Zolotukhin S. Identification of low-expressing transcripts of the NPY receptors’ family in the murine lingual epithelia. Methods Mol Biol. 2014;1211:213-28.
  10. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M, Cleaver B, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant Adeno-associated virus (rAAV) vectors. Mol Ther.  Methods, 2014, 1, 14034.
  11. Marsic D, Govindasamy L, Currlin S, Markusic DM, Tseng YS, Herzog RW, Agbandje-McKenna M, and Zolotukhin S. Vector design tour de force: integrating combinatorial and rational approaches to derive novel Adeno-associated virus (AAV) variants. Mol Ther. 2014 Nov;22(11):1900-9.
  12. Mietzsch M, Grasse S, Zurawski C, Weger S, Bennett A, Agbandje-McKenna M, Muzyczka N, Zolotukhin S, Heilbronn R. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy Hum Gene Ther. 2014 Mar;25(3):212-22.
  13. Hurtado MD, Sergeyev V, Acosta A, Spegele M, La Sala M, Herzog H, Dotson CD, Gorbatyuk O, Zolotukhin S. Salivary PYY3-36 modulates ingestive behavior without inducing taste aversion. The J. of Neuroscience, 2013, 33(47):18368 –18380.
  14. La Sala MS, Hurtado MD, Brown AR, Bohórquez DV, Liddle RA, Herzog H, Zolotukhin S, Dotson CD. Modulation of taste responsiveness by the satiation hormone peptide YY. FASEB J. 2013; 27(12):5022-33.
  15. Zolotukhin S. Metabolic hormones in saliva: origins and functions. Invited Reviews. Oral Diseases, 2013, V. 13, issue, 3, pp. 219-229.
  16. Hurtado MD,  Acosta A,  Riveros PP, Baum BJ, Ukhanov K, Brown AR, Dotson CD, Herzog H, and Zolotukhin S, Y-receptors’ distribution in murine lingual epithelia. PLoS ONE, 2012;7(9):e46358.
  17. Acosta A, Hurtado MD, Gorbatyuk O, La Sala M, Duncan D, Aslanidi G, Campbell-Thompson M, Zhang L, Herzog H, Voutetakis A, Baum BJ, Zolotukhin S. Salivary PYY: A Putative Bypass to Satiety. PLoS ONE 2011; 6(10):e26137.
  18. *Aslanidi G, *Lamb K, Zolotukhin S. An inducible system for highly efficient production of recombinant adeno-associated virus (rAAV) vectors in insect Sf9 cells. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5059-64.
  19. Zhong L, Li B, Mah CS, Govindasamy L, Agbandje-McKenna M, Cooper M, Herzog RW, Zolotukhin I, Warrington KH Jr, Weigel-Van Aken KA, Hobbs JA, Zolotukhin S, Muzyczka N, and Srivastava A. Next generation of adeno-associated virus 2 vectors: Point mutations in tyrosine lead to high-efficiency transduction at lower doses. Proc Natl Acad Sci U S A. 2008 June;105 (3):7827–7832.